PoplarV1, Main, Exploration, bibRecord, 001134

Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD).

Identifieur interne : 001134 ( Main/Exploration ); précédent : 001133; suivant : 001135

Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD).

Auteurs : Merve Özparpucu [Suisse] ; Markus Rüggeberg [Suisse] ; Notburga Gierlinger [Autriche] ; Igor Cesarino [Brésil] ; Ruben Vanholme [Belgique] ; Wout Boerjan [Belgique] ; Ingo Burgert [Suisse]

Source :

The Plant journal : for cell and molecular biology [ 1365-313X ] ; 2017.

RBID : pubmed:28440915

Descripteurs français

English descriptors

KwdEn :
- Alcohol Oxidoreductases (genetics), Alcohol Oxidoreductases (metabolism), Cell Wall (genetics), Cell Wall (metabolism), Lignin (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Spectroscopy, Fourier Transform Infrared (MeSH).
MESH :
- chemical , genetics : Alcohol Oxidoreductases, Plant Proteins.
- chemical , metabolism : Alcohol Oxidoreductases, Lignin, Plant Proteins.
- genetics : Cell Wall, Plants, Genetically Modified, Populus.
- metabolism : Cell Wall, Plants, Genetically Modified, Populus.
- Spectroscopy, Fourier Transform Infrared.

Abstract

Lignin engineering is a promising tool to reduce the energy input and the need of chemical pre-treatments for the efficient conversion of plant biomass into fermentable sugars for downstream applications. At the same time, lignin engineering can offer new insight into the structure-function relationships of plant cell walls by combined mechanical, structural and chemical analyses. Here, this comprehensive approach was applied to poplar trees (Populus tremula × Populus alba) downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD) in order to gain insight into the impact of lignin reduction on mechanical properties. The downregulation of CAD resulted in a significant decrease in both elastic modulus and yield stress. As wood density and cellulose microfibril angle (MFA) did not show any significant differences between the wild type and the transgenic lines, these structural features could be excluded as influencing factors. Fourier transform infrared spectroscopy (FTIR) and Raman imaging were performed to elucidate changes in the chemical composition directly on the mechanically tested tissue sections. Lignin content was identified as a mechanically relevant factor, as a correlation with a coefficient of determination (r²) of 0.65 between lignin absorbance (as an indicator of lignin content) and tensile stiffness was found. A comparison of the present results with those of previous investigations shows that the mechanical impact of lignin alteration under tensile stress depends on certain structural conditions, such as a high cellulose MFA, which emphasizes the complex relationship between the chemistry and mechanical properties in plant cell walls.

DOI: 10.1111/tpj.13584
PubMed: 28440915

Affiliations:

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Le document en format XML

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<term>Cell Wall (metabolism)</term>
<term>Lignin (metabolism)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (metabolism)</term>
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<term>Populus (metabolism)</term>
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<term>Alcohol oxidoreductases (métabolisme)</term>
<term>Lignine (métabolisme)</term>
<term>Paroi cellulaire (génétique)</term>
<term>Paroi cellulaire (métabolisme)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Spectroscopie infrarouge à transformée de Fourier (MeSH)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
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<term>Plant Proteins</term>
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<term>Paroi cellulaire</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term>
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<term>Paroi cellulaire</term>
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<term>Protéines végétales</term>
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<front><div type="abstract" xml:lang="en">Lignin engineering is a promising tool to reduce the energy input and the need of chemical pre-treatments for the efficient conversion of plant biomass into fermentable sugars for downstream applications. At the same time, lignin engineering can offer new insight into the structure-function relationships of plant cell walls by combined mechanical, structural and chemical analyses. Here, this comprehensive approach was applied to poplar trees (Populus tremula × Populus alba) downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD) in order to gain insight into the impact of lignin reduction on mechanical properties. The downregulation of CAD resulted in a significant decrease in both elastic modulus and yield stress. As wood density and cellulose microfibril angle (MFA) did not show any significant differences between the wild type and the transgenic lines, these structural features could be excluded as influencing factors. Fourier transform infrared spectroscopy (FTIR) and Raman imaging were performed to elucidate changes in the chemical composition directly on the mechanically tested tissue sections. Lignin content was identified as a mechanically relevant factor, as a correlation with a coefficient of determination (r²) of 0.65 between lignin absorbance (as an indicator of lignin content) and tensile stiffness was found. A comparison of the present results with those of previous investigations shows that the mechanical impact of lignin alteration under tensile stress depends on certain structural conditions, such as a high cellulose MFA, which emphasizes the complex relationship between the chemistry and mechanical properties in plant cell walls.</div>
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<Abstract><AbstractText>Lignin engineering is a promising tool to reduce the energy input and the need of chemical pre-treatments for the efficient conversion of plant biomass into fermentable sugars for downstream applications. At the same time, lignin engineering can offer new insight into the structure-function relationships of plant cell walls by combined mechanical, structural and chemical analyses. Here, this comprehensive approach was applied to poplar trees (Populus tremula × Populus alba) downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD) in order to gain insight into the impact of lignin reduction on mechanical properties. The downregulation of CAD resulted in a significant decrease in both elastic modulus and yield stress. As wood density and cellulose microfibril angle (MFA) did not show any significant differences between the wild type and the transgenic lines, these structural features could be excluded as influencing factors. Fourier transform infrared spectroscopy (FTIR) and Raman imaging were performed to elucidate changes in the chemical composition directly on the mechanically tested tissue sections. Lignin content was identified as a mechanically relevant factor, as a correlation with a coefficient of determination (r²) of 0.65 between lignin absorbance (as an indicator of lignin content) and tensile stiffness was found. A comparison of the present results with those of previous investigations shows that the mechanical impact of lignin alteration under tensile stress depends on certain structural conditions, such as a high cellulose MFA, which emphasizes the complex relationship between the chemistry and mechanical properties in plant cell walls.</AbstractText>
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<ForeName>Merve</ForeName>
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</AffiliationInfo>
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</AffiliationInfo>
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<AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Laboratory of Applied Wood Materials, Empa, 8600, Dübendorf, Switzerland.</Affiliation>
</AffiliationInfo>
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<ForeName>Notburga</ForeName>
<Initials>N</Initials>
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</AffiliationInfo>
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<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, (Technologiepark 927), 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boerjan</LastName>
<ForeName>Wout</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Department of Plant Biotechnology and Bioinformatics, Technologiepark 927, Ghent University, 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>VIB Center for Plant Systems Biology, (Technologiepark 927), 9052, Ghent, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Burgert</LastName>
<ForeName>Ingo</ForeName>
<Initials>I</Initials>
<AffiliationInfo><Affiliation>Institute for Building Materials (IfB), ETH Zurich, 8093, Zurich, Switzerland.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Laboratory of Applied Wood Materials, Empa, 8600, Dübendorf, Switzerland.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>Y 728</GrantID>
<Agency>Austrian Science Fund FWF</Agency>
<Country>Austria</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2017</Year>
<Month>06</Month>
<Day>25</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Plant J</MedlineTA>
<NlmUniqueID>9207397</NlmUniqueID>
<ISSNLinking>0960-7412</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9005-53-2</RegistryNumber>
<NameOfSubstance UI="D008031">Lignin</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.1.-</RegistryNumber>
<NameOfSubstance UI="D000429">Alcohol Oxidoreductases</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.1.1.195</RegistryNumber>
<NameOfSubstance UI="C018656">cinnamyl alcohol dehydrogenase</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000429" MajorTopicYN="N">Alcohol Oxidoreductases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus tremula × Populus alba</Keyword>
<Keyword MajorTopicYN="N">cell wall mechanics, lignin</Keyword>
<Keyword MajorTopicYN="N">cinnamyl alcohol dehydrogenase</Keyword>
<Keyword MajorTopicYN="N">genetic modification</Keyword>
<Keyword MajorTopicYN="N">microtensile test</Keyword>
<Keyword MajorTopicYN="N">plant cell wall</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year>
<Month>03</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2017</Year>
<Month>04</Month>
<Day>18</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>4</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>12</Month>
<Day>19</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>4</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28440915</ArticleId>
<ArticleId IdType="doi">10.1111/tpj.13584</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Autriche</li>
<li>Belgique</li>
<li>Brésil</li>
<li>Suisse</li>
</country>
<region><li>Province de Flandre-Orientale</li>
<li>Région flamande</li>
<li>Vienne (Autriche)</li>
<li>État de São Paulo</li>
</region>
<settlement><li>Gand</li>
<li>São Paulo</li>
<li>Vienne (Autriche)</li>
</settlement>
<orgName><li>Université de Gand</li>
<li>Université de São Paulo</li>
</orgName>
</list>
<tree><country name="Suisse"><noRegion><name sortKey="Ozparpucu, Merve" sort="Ozparpucu, Merve" uniqKey="Ozparpucu M" first="Merve" last="Özparpucu">Merve Özparpucu</name>
</noRegion>
<name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name>
<name sortKey="Burgert, Ingo" sort="Burgert, Ingo" uniqKey="Burgert I" first="Ingo" last="Burgert">Ingo Burgert</name>
<name sortKey="Ozparpucu, Merve" sort="Ozparpucu, Merve" uniqKey="Ozparpucu M" first="Merve" last="Özparpucu">Merve Özparpucu</name>
<name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name>
<name sortKey="Ruggeberg, Markus" sort="Ruggeberg, Markus" uniqKey="Ruggeberg M" first="Markus" last="Rüggeberg">Markus Rüggeberg</name>
</country>
<country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Gierlinger, Notburga" sort="Gierlinger, Notburga" uniqKey="Gierlinger N" first="Notburga" last="Gierlinger">Notburga Gierlinger</name>
</region>
</country>
<country name="Brésil"><region name="État de São Paulo"><name sortKey="Cesarino, Igor" sort="Cesarino, Igor" uniqKey="Cesarino I" first="Igor" last="Cesarino">Igor Cesarino</name>
</region>
</country>
<country name="Belgique"><region name="Région flamande"><name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name>
</region>
<name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name>
<name sortKey="Boerjan, Wout" sort="Boerjan, Wout" uniqKey="Boerjan W" first="Wout" last="Boerjan">Wout Boerjan</name>
<name sortKey="Vanholme, Ruben" sort="Vanholme, Ruben" uniqKey="Vanholme R" first="Ruben" last="Vanholme">Ruben Vanholme</name>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD).
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Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD).

Unravelling the impact of lignin on cell wall mechanics: a comprehensive study on young poplar trees downregulated for CINNAMYL ALCOHOL DEHYDROGENASE (CAD).

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